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SECOND INTERNATIONAL SYMPOSIUM
ON THE ROLE OF SOY
IN PREVENTING AND TREATING CHRONIC DISEASESeptember 15-18, 1996
Brussells, Belgium
SCIENTIFIC PROGRAM
(Oral Abstracts)Soy and Heart Disease : Effects Independent of Cholesterol Reduction
ANTI-Atherogenic Effects of Soybean Protein. Viewpoints from Peroxidizability and Molecular Size of LDL and From Anti-Platelet Aggregation
Takemichi Kanazawa.
The Second Department of Internal Medicine, Hirosaki University School of Medicine, Hirosaki, Japan.To investigate the biological function of soy protein for prevention of vascular diseases, the following studies were carried out.
Low density lipoprotein (LDL) was separated from plasma of the patient with myocardial infarction (22 cases), cerebral infarction (18 cases), familial hypercholesterolemia (8 cases) and healthy (18 persons) by ultracentrifugation. Peroxidized LDL was evaluated by estimation of peroxidized cholesteryl linoleate on thin layer chromatography or of lipid peroxide by Determiner Kit. Lipids were measured by enzymatic method. Molecular structure of soybean peptide was determined by IH-NMR, HPLC and MS. Molecular size of LDL was calculated by planimeter from photograph of negative stain. Soy creme contained 10.6% protein was prepared by our previous method (J. Nutr., 125, 639, 1995). Soybean peptide was separated by protease treatment, ODS-AQ column chromatography and HPLC.
1. Normal LDL obtained from healthy persons did not enhanced platelet aggregation induced by ADP, collagen and epinephrine, but LDL of patient with vascular disease enhanced clearly platelet aggregation.
2. Administration of soy creme suppressed markedly peroxidizability of LDL due to Cu++ (in rabbit).
3. Soy creme administered orally suppressed an enlargement of LDL during cholesterol feeding (in rabbit).
4. Soy creme administration reduced LDL cholesterol level (in human and rabbit).
5. A peptide consisted of six kinds of amino acids which was prepared from soybean suppressed markedly platelet aggregation, and composition and structure of amino acid was ASP-Glu-Gly-Leu-Phe-Arg.
Conclusion. Various molecular constituents with useful function such as anti-peroxidizability to LDL, anti-platelet aggregability, suppression of enlargement of LDL molecular size during cholesterol administration and cholesterol reducing effect were contained in soybean. Thus, soybeans are very useful food for preventing vascular diseases.
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