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SECOND INTERNATIONAL SYMPOSIUM
ON THE ROLE OF SOY
IN PREVENTING AND TREATING CHRONIC DISEASESeptember 15-18, 1996
Brussells, Belgium
POSTER ABSTRACTSGROWTH INHIBITION OF HUMAN PROSTATIC CELL LINES BY PHYTO-OESTROGENS
Hempstock J., Kavanagh J.P. & George N.J.R.
Department of Urology, South Manchester University Hospitals Trust, Withington,
Manchester, M20 2LR, UK.
The incidence of prostatic and other hormonally dependent cancers is low in populations with a high dietary intake of soy products. It has been suggested that phyto-oestrogens derived from soy have a role in preventing the development and spread of these tumours. This study focuses on possible effects of some phyto-oestrogens on the proliferation of three human prostatic cell lines; PC3 cells, derived from a grade 4 adenocarcinoma, PNT1A and PNT2 cells, from normal prostatic epithelium, immortalised with SV40. 48 hours after seeding at a density of 10000 cells per well in 96 well plates, the cells were exposed to phyto-oestrogens at various concentrations for 72 hours. Proliferation was assessed by incorporation of BrdU over 4 hours, measured using an ELISA technique. Results are expressed as % of control values. Biochanin A, daidzein, genistein and genistin all gave dose dependent growth
inhibition with each of the cell lines. At 20 umol/l biochanin A and daidzein had little effect on PC3 cells while genistein and genistin reduced BrdU uptake to about 40% of controls. The PNT1A cells were unaffected by biochanin A at 20 umol/l whereas daidzein, genistein and genistin reduced proliferation to 78%, 86% and 51%, respectively. PNT2 cells were more responsive than PNT1A cells at 20 umol/l (growth compared to controls was 71%, 53%, 74%
and 42% with biochanin A, daidzein, genistein and genistin, respectively). At 100 umol/l, growth was effectively abolished for each cell line by biochanin A, genistein and genistin; daidzein reduced values to 45% (PC3), 56% (PNT2) and 24% (PNT1A). These phyto-oestrogens are therefore effective growth inhibitors of a prostatic carcinoma cell line and two non-cancerous epithelial derived prostate cell lines. This is further evidence supporting the hypothesised role of these dietary products in prevention and development of prostatic cancer.
This work was supported by the World Cancer Research Fund.
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